Preparation of injections with fungal spores

Materials needed:

• sterile syringe with needle
• sterile water
• alcohol or similar burner
• low glass or metal container with lid
• ethanol or similar disinfectant

Cut off the mushroom cap just at the junction with the beta and place it in a low dish (Figure 1) that has been previously cleaned with alcohol or sterilised at 180ºC for 30 minutes. Leave the hat in the container for a few hours to allow the spores to accumulate on the bottom – the spore footprint (Figure 2).

Figure 1: Shiitake mushroom cap in a container